Carbon nanotube-coated recombinant human surfactant protein D reduces cell viability in an ovarian cancer cell line, SKOV3, and modulates mTOR pathway and pro-inflammatory cytokine response

Nanoparticles such as carbon nanotubes (CNTs) have various clinical and diagnostic applications utilised for imaging drug delivery. Therapeutic proteins/peptides can be loaded on CNT coronas to specifically hit immune cells in overdrive or uncontrolled malignant cells. Previously, it was reported that a recombinant version of human surfactant protein D (rfhSP-D) containing trimeric C-type lectin domains induced apoptosis several tumour cells/cell lines, including SKOV3, which is an ovarian cell line. Solid-phase rfhSP-D coated microtiter plate considerably more potent inducing breast We immobilised highly purified, endotoxin-free CNTs assessed its antiproliferative effect SKOV3 Biotinylated rfhSP-D-CNTs were phagocytosed by cells, followed time-dependent manner. Gene expression analysis revealed compromised mTOR complex mechanism apoptosis. When added culture system produced proinflammatory response likely anti-tumorigenic. Thus, rfhSP-D-CNT seems worthwhile nanocarrier testing vivo using animal model orthotopic cancer.